Open AccessComparison of assays for Cryptosporidium parvum oocysts viability after chemical disinfection
Author(s) -
Black E. Kathleen,
Finch Gordon R,
TaghiKilani Ruhi,
Belosevic Miodrag
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb07987.x
Subject(s) - infectivity , cryptosporidium parvum , propidium iodide , dapi , microbiology and biotechnology , viability assay , in vitro , biology , staining , cryptosporidium , chemistry , virology , biochemistry , feces , virus , apoptosis , genetics , programmed cell death
In vitro excystation, vital dyes (4′, 6‐diamidino‐2‐phenylindole (DAPI) and propidium iodide (PI)), and infeictivity in neonatal CD‐1 mice were used to assess the viability of Cryptosporidium parvum oocysts after chemical disinfection. In vitro excystation and DAPI/PI staining provided similar estimates of viability in bench‐scale experiments, but both of these methods significantly overestimated the viability when compared with infectivity (Pr ≤ 0.01). Infectivity was the most reliable measure of the viability of C. parvum oocysts following chemical disinfection.