Open AccessTransposons Tn916 and Tn925 can transfer from Enterococcus faecalis to Leuconostoc oenos
Author(s) -
Zúñiga Manuel,
Pardo Isabel,
Ferrer Sergi
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb07986.x
Subject(s) - leuconostoc , biology , enterococcus faecalis , transposable element , lactococcus lactis , genetics , lactococcus , microbiology and biotechnology , streptococcaceae , enterococcus , bacteria , genome , staphylococcus aureus , gene , lactic acid
The streptococcal transposons Tn916 and Tn925 were transferred to several strains of Leuconostoc (Ln.) oenos using the filter mating method. The insertion of both transposons into the chromosome occurred at different sites. Transconjugants of Ln. oenos carrying Tn916 could serve as donors in mating experiments with Lactococcus lactis LM2301. Further analysis of L. lactis LM2301 transconjugants showed that the insertion of the transposon Tn916 into the chromosome was site‐specific. These studies establish a basis for the initiation of genetic studies in this Leuconostoc species since there are no efficient conjugal or transformation systems previously described for this microorganism.