Open AccessA Bacillus subtilis 168 mutant with increased xylose uptake can utilize xylose as sole carbon source
Author(s) -
Schmiedel Dagmar,
Hillen Wolfgang
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb07985.x
Subject(s) - xylose , bacillus subtilis , carbon source , mutant , operon , biochemistry , chemistry , sugar , bacteria , microbiology and biotechnology , biology , fermentation , gene , genetics
Bacillus subtilis 168 is unable to effectively utilize xylose as sole carbon source. We demonstrate here that this strain cannot actively transport xylose into the cell. After leaving B. subtilis 168 for a few days on minimal plates with xylose as sole carbon source large colonies arise with a frequency of 1 × 10 −6 /cell. These mutants grow well on xylose and efficiently take up that sugar. This new property is not inducible by xylose, indicating that the mutation is neither in the xyl nor in the xyn operon.