Open AccessCloning and expression of the Mycobacterium fortuitum Superoxide dismutase gene
Author(s) -
Menéndez M. Carmen,
Domenech Pilar,
Prieto Jesus,
García María J.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07950.x
Subject(s) - superoxide dismutase , mycobacterium fortuitum , cloning (programming) , gene , biology , microbiology and biotechnology , gene expression , molecular cloning , enzyme , biochemistry , genetics , mycobacterium , bacteria , computer science , programming language
In this paper we report the cloning, sequencing and expression of the Superoxide dismutase ( sod ) gene from Mycobacterium fortuitum . A single gene was found to code for Superoxide dismutase activity with its identity being confirmed by expression in M. aurum . The amino acid sequence was found to be similar to that of Superoxide dismutases of several other origins. A region downstream of the sod gene also showed similarities to the corresponding sequences of the two main mycobacterial pathogens: M. leprae and M. tuberculosis . Analysis of enzymatic activity showed this enzyme in M. fortuitum required manganese as cofactor.