Open AccessCloning and sequence analysis of Vibrio parahaemolyticus ompK gene encoding a 26‐kDa outer membrane protein, OmpK, that serves as receptor for a broad‐host‐range vibriophage, KVP40
Author(s) -
Inoue Tetsuyoshi,
Matsuzaki Shigenobu,
Tanaka Shuji
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07945.x
Subject(s) - vibrio parahaemolyticus , biology , gene , nucleic acid sequence , peptide sequence , amino acid , aeromonas hydrophila , sequence analysis , molecular cloning , bacterial outer membrane , signal peptide , vibrio , cloning (programming) , genetics , microbiology and biotechnology , bacteria , escherichia coli , computer science , programming language
The ompK gene of Vibrio parahaemolyticus 1010 (RIMD 2210001) encoding an outer membrane protein (OMP), OmpK, which serves as the receptor for a broad‐host‐range vibriophage, KVP40, was cloned and sequenced. The gene consisted of 789 nucleotides encoding 263 amino acids. Since the first 20 amino acids most likely constitute the signal peptide, mature OmpK would consist of 243 amino acids with a calculated molecular mass of 27458 Da. Sequence comparisons indicate that OmpK is unique among Vibrio OMPs so far sequenced, but may be distantly related to Tsx of enteric bacteria and is homologous to an Aeromonas hydrophila OMP, protein IV.