Open AccessParasporal body from Bacillus thuringiensis subsp. kenyae composed of a novel combination of inclusions and Cry proteins
Author(s) -
Lopezmeza Joel E.,
Federici Brian A.,
Johnson Jeffrey J.,
Ibarra Jorge E.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07937.x
Subject(s) - bacillus thuringiensis , plutella , subspecies , biology , bacillales , bacillaceae , microbiology and biotechnology , botany , bacteria , genetics , larva , zoology , bacillus subtilis
A new type of insecticidal parasporal body is reported from Bacillus thuringiensis subspecies kenyae (H4a4c) isolated from maize dust in Mexico. This parasporal body is spherical to oblong and composed of three inclusions, one spherical, one cuboidal, and one bipyramidal. Together, these contain four major proteins, three in the Cryl size class (138, 133, and 131 kDa), and one in the CryII size class (65 kDa). Monoclonal antibodies that recognize CryIB reacted with the 138‐kDa protein, but those that recognize CryIA(a) or CryIA(a) and CryIA(b) did not react with any of the proteins. This isolate was more toxic (LC 50 = 96 ng cm −2 diet) to first instars of the diamond back moth, Plutella xylostella , than the HD1 isolate of B. thuringiensis subsp. kurstaki ( LC 50 = 182 ng cm −2 diet).