Open AccessInduction of macrophage apoptosis by Bordetella pertussis adenylate cyclase‐hemolysin
Author(s) -
Khelef Nadia,
Guiso Nicole
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07909.x
Subject(s) - bordetella pertussis , adenylate cyclase toxin , pertactin , filamentous haemagglutinin adhesin , pertussis toxin , microbiology and biotechnology , adenylate kinase , bacterial adhesin , cyclase , biology , g protein , biochemistry , virulence , receptor , signal transduction , bacteria , gene , genetics
We previously demonstrated that Bordetella pertussis induced macrophage apoptosis and that adenylate cyclase‐hemoFlysin expression is required for this phenomenom. In order to determine a role for other B. pertussis factors, such as pertussis toxin or the adhesins, pertactin and filamentous hemagglutinin, we compared mutants deficient in adhesin expression or purified proteins for their ability to induce cell death. We showed that only purified adenylate cyclase‐hemolysin, but neither adhesins, nor pertussis toxin, killed macrophages through apoptosis. We also demonstrated that all activities of adenylate cyclase‐hemolysin were required. Moreover, our results indicate that B. pertussis does not have to invade cells to be lethal.