Open AccessRecovery of a bacterial sub‐population from sewage using immunofluorescent flow cytometry and cell sorting
Author(s) -
Porter Jonathan,
Robinson Joan,
Pickup Roger,
Edwards Clive
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07883.x
Subject(s) - flow cytometry , cell sorting , population , microbiology and biotechnology , biology , agar , sewage , immunofluorescence , agar plate , cytometry , bacteria , chemistry , antibody , immunology , environmental science , genetics , environmental engineering , medicine , environmental health
The effectiveness of immunofluorescence flow cytometry and cell sorting to detect, quantify and separate indigenous bacterial populations present in low concentrations in sewage outflow was investigated. Preparatory experiments for targeted recovery revealed indigenous, immunoglobulin‐G‐binding particles present at low levels in sewage outflow samples taken from Coniston Water. Fluorescence‐activated cell sorting of this population was employed to enrich for these particles, which were confirmed as bacterial cells. This cell population comprised approximately 23% of the total plate count on MacConkey agar before cell sorting, rising to approximately 95% after sorting. These results corresponded to cell densities of less than 5% of the total plate count on R2A agar. Taxonomic tests suggested the bacterium to be Ochrobactrum anthropi .