Open AccessAnalysis of S‐layer proteins of Lactobacillus brevis
Author(s) -
Yasui Tetsuji,
Yoda Kumiko,
Kamiya Takayuki
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07881.x
Subject(s) - lactobacillus brevis , western blot , s layer , antiserum , microbiology and biotechnology , biology , food spoilage , layer (electronics) , lactobacillus , chemistry , bacteria , biochemistry , genetics , gene , antibody , lactic acid , organic chemistry , lactobacillus plantarum
Abstract The presence of S‐layer proteins in Lactobacillus brevis was examined by SDS‐PAGE analysis. Thirty six out of a total of 41 L. brevis strains possessed S‐layer proteins of molecular masses ranging from 38 to 55 kDa. Western blot analysis using antisera raised against whole cells of S‐layer protein‐carrying strains demonstrated the heterogeneity of L. brevis S‐layer proteins. No clear relationship was observed between the presence of S‐layer proteins or their immunological characteristics and the physiological activity of L. brevis as a beer spoilage organism.