Open AccessAberrant gene for El Tor hemolysin from Vibrio cholerae non‐01, N037
Author(s) -
Honma Yasuko,
Yamamoto Koichiro,
Iwanaga Masaaki
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07876.x
Subject(s) - vibrio cholerae , hemolysin , el tor , microbiology and biotechnology , vibrionaceae , biology , gene , cholera , genetics , bacteria , virulence
Vibrio cholerae non‐O1 strain N037 produced a hemolysin (N037‐Hly) which was antigenically similar to El Tor hemolysin (El Tor‐Hly) but different in molecular size, hemolytic activity, and glucose binding capacity. In the gene encoding N037‐Hly, a 4‐bp insertion into the structural gene for El Tor‐Hly ( hlyA ) was found. The insertion resulted in a shift of codon frames generating a new stop codon in the downstream region. N037‐Hly was a truncated product of El Tor‐Hly sharing 90% of the N terminal region. This suggested that the 10% C‐terminal region of El Tor‐Hly is needed for the maximal hemolytic activity and glucose binding capacity.