Open AccessIdentification of the replication region of Lactobacillus acidophilus plasmid pLA103
Author(s) -
Kanatani Kazuo,
Tahara Takatsugu,
Oshimura Masao,
Sano Keiji,
Umezawa Chisae
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07872.x
Subject(s) - lactobacillus acidophilus , plasmid , biology , open reading frame , genetics , peptide sequence , homology (biology) , nucleic acid sequence , sequence analysis , dna , gene , bacteria , probiotic
The structure of the region necessary for replication of the plasmid pLA103 from Lactobacillus acidophilus TK8912 has been characterized. Sequence analysis revealed that the replication region contained an open reading frame (OrfA) encoding a 282‐amino acid peptide preceded by a 22‐bp tandem repeat sequence region. The predicted OrfA protein showed homology to the replication protein of a plasmid from Pediococcus halophilus . The plasmid containing the repeat sequence region preceding OrfA was able to replicate in the Lactobacillus host when provided with OrfA in trans , suggesting that the repeat sequence region contains the origin sequence essential for the pLA103 replication.