Open AccessRapid identification of Acremonium lolii and Acremonium coenophialum endophytes through arbitrarily primed PCR
Author(s) -
Liua D.,
Heeswijck R.,
Latch G.,
Leonforte T.,
Panaccio M.,
Langford C.,
Cunningham P.,
Reed K.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07867.x
Subject(s) - acremonium , biology , botany
Using a random decamer 5′‐CCGAGGTGAC‐3′ in an arbitrarily primed PCR, similar band patterns were observed between Acremonium lolii and A. coenophialum DNA, which were somewhat different from those formed by other fungal DNA. Despite sharing bands of around 0.7, 0.9 and 2.1 kb, A. lolii can be distinguished from A. coenophialum by the presence of an additional band at around 0.5 kb in the arbitrarily primed PCR.