Open AccessTranscriptional analysis of the β‐galactosidase gene ( pbg ) in Clostridium perfringens
Author(s) -
Kobayashi Takumi,
Shimizu Tohru,
Hayashi Hideo
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07862.x
Subject(s) - operon , clostridium perfringens , l arabinose operon , gal operon , lac operon , gene , biology , trp operon , microbiology and biotechnology , catabolite repression , gene expression , genetics , escherichia coli , bacteria , mutant
The mode of expression of the β‐galactosidase gene ( pbg ) of Clostridium perfringens was examined. The pbg gene was transcribed on a single 3.7‐kb mRNA. The transcript contained a message for ORF54, located upstream of the pbg gene in the chromosome, indicating that ORF54 and the pbg gene comprise one operon ( pbg operon). Expression of the pbg operon was induced by lactose at the transcriptional level. The promoter structure of the pbg operon was characterized by many palindrome structures and direct repeats, which suggests that there might be some catabolite regulation of the expression of the pbg operon in C. perfringens .