Open AccessSite‐specific restriction endonucleases in Bacillus licheniformis
Author(s) -
Parini Carlo,
Fortina M. Grazia
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07847.x
Subject(s) - isoschizomer , bacillus licheniformis , restriction enzyme , endonuclease , biology , strain (injury) , dna , bacillaceae , genetics , recognition sequence , restriction fragment , microbiology and biotechnology , bacteria , gene , bacillus subtilis , gene expression , anatomy , dna methylation
We systematically studied site‐specific restriction endonucleases in Bacillus licheniformis strains and detected endonuclease activity in 25 of 217 strains tested. Three different activities were obtained. One of these activities detected in 21 strains was the most representative within the species and produced a banding pattern, after digestion of A DNA, identical to that seen with Cla I. Two other strains isolated from soil samples from China and USA were found to produce a DNA‐cleaving enzyme with the same recognition sequence as Bsa I. One producer strain, isolated from a Peruvian soil sample, showed to possess a mixture of two isoschizomers, Cla I and Bsa I. Finally, one strain produced an endonuclease activity, not previously described in B. licheniformis , that showed the same recognition sites as Bsu 361.