Open AccessThe aniline blue fluorochrome specifically stains the septum of both live and fixed Schizosaccharomyces pombe cells
Author(s) -
Kippert Fred,
Lloyd David
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07836.x
Subject(s) - schizosaccharomyces pombe , staining , stain , aniline , cytoplasm , chemistry , yeast , differential staining , vital stain , schizosaccharomyces , biochemistry , biology , saccharomyces cerevisiae , organic chemistry , genetics
A novel method is described which uses aniline blue for the specific fluorescent staining of the septa of dividing cells of the fission yeast, Schizosaccharomvces pombe . It gives the same results with live and fixed cells. In fixed or, more generally, dead cells there is no staining of the cytoplasm: this renders aniline blue superior to other dyes previously used to stain the septum of S. pombe . This feature allows quantitative analysis of the septum index for fixed samples and, therefore, makes aniline blue the stain of choice for cell cycle kinetic studies.