Open AccessFormation of 2‐hydroxy‐4‐methylpentanoic acid from l‐leucine by Clostridium butyricum
Author(s) -
Butel MarieJosé,
Rimbault Alain,
Khelifa Nasser,
Campion Geneviève,
Szylit Odette,
Rocchiccioli Francis
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07828.x
Subject(s) - clostridium butyricum , leucine , yeast extract , metabolite , biochemistry , chemistry , chromatography , amino acid , biology , fermentation
Five Clostridium butyricum strains of different origin were grown in trypticase‐yeast extract‐hemin medium with or without d‐glucose (TGYH or TYH medium, respectively) and in a synthetic basal medium with d‐glucose (BMG medium). 2‐Hydroxy‐4‐methylpentanoic acid was detected by gas chromatography‐mass spectrometry (GC‐MS) for the five strains whether grown in TGYH or TYH medium (270 or 170 μM, respectively). In BMG medium supplemented with l‐leucine (10 mM), the concentration of this metabolite was strongly increased (2.8 mM versus 10 μM in the control). After culture in TGYH or TYH medium supplemented with l‐( methyl ‐ 2 H 3 )leucine, 2‐hydroxy‐4‐([ 2 H 3 ]methyl)pentanoic acid was detected by GC‐MS. This observation demonstrates that C. butyricum is able to convert l‐leucine into the corresponding 2‐hydroxy acid and opens a new aspect in the study of C. butyricum metabolism.