Open AccessRapid identification of Streptococcus pneumoniae by PCR amplification of ribosomal DNA spacer region
Author(s) -
Saruta Katsutoshi,
Matsunaga Teiichi,
Hoshina Sadayori,
Kono Midori,
Kitahara Shintaro,
Kanemoto Sonomi,
Sakai Osamu,
Machida Katsuhiko
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07827.x
Subject(s) - streptococcus pneumoniae , polymerase chain reaction , ribosomal rna , microbiology and biotechnology , biology , ribosomal dna , dna , 23s ribosomal rna , 16s ribosomal rna , streptococcus , gene , bacteria , genetics , rna , ribosome , phylogenetics , antibiotics
Streptococcus pneumoniae is one of the important human pathogens in clinical microbiology. A polymerase chain reaction assay was designed to detect and identify S. pneumoniae through amplification of the ribosomal DNA spacer regions between the pneumococcal 16S‐23S ribosomal RNA genes. Thirty‐two Streptococcus and non‐ Streptococcus strains were tested to verify the specificity of the assay, and only S. pneumoniae strains gave a positive reaction. This method is a powerful technique for the rapid identification of S. pneumoniae .