Open AccessEffects of glucose, tetrapyrroles and protein kinase C activators on cell proliferation in cultures of Saccharomyces cerevisiae
Author(s) -
Overgaard A.K.,
Friis J.,
Christensen L.,
Christiansen H.,
Rasmussen L.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07826.x
Subject(s) - biochemistry , chlorophyllin , saccharomyces cerevisiae , hemin , cell growth , protein kinase c , protoporphyrin ix , biology , protein kinase a , glycerol , yeast , glycerol kinase , protoporphyrin , kinase , chemistry , enzyme , heme , chlorophyll , porphyrin , botany , photodynamic therapy , organic chemistry
Saccharomyces cerevisiae was inoculated into a yeast nitrogen base with either glycerol or glucose as carbon source. Cell proliferation was followed by colony counts on agar medium. Cells in the glycerol‐supplemented medium divided less than once in 10 days. When glucose, 6‐deoxy‐glucose or protoporphyrin IX was added, the cells had doubling times of about 24 h and increased in number to about 0.5 × 10 6 cells ml −1 Addition of either of the protein kinase C activators oleoyl‐acetylglycerol or phorbol‐12‐myristate‐13‐acetate did not activate cell proliferation in the glycerol medium. However, when (i) glucose was combined with either protoporphyrin IX or chlorophyllin, or (ii) either protoporphyrin IX or chlorophyllin was combined with either of the protein kinase C activators, the cells had doubling times of about 12 h. Hence, (i) glucose can act as both a carbon source and a signalling molecule for proliferation, and (ii) two systems are involved in activating cell proliferation in S. cerevisiae : one operating through a protein kinase C system and another through a guanylate cyclase system.