Open AccessAnalysis of the minor autolysins of Bacillus subtilis during vegetative growth by zymography
Author(s) -
Rashid Muhammad Harunur,
Sato Naoko,
Sekiguchi Junichi
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07822.x
Subject(s) - autolysin , bacillus subtilis , zymography , biology , microbiology and biotechnology , autolysis (biology) , biochemistry , mutation , gene , mutant , enzyme , genetics , bacteria
SDS‐PAGE and zymographic analysis of protein extracts from Bacillus subtilis AN8, which is deficient in the major 50‐kDa amidase (CwlB[LytC]), revealed another distinct but relatively weak 50‐kDa protein and its strong activity band. As well as the 50‐kDa protein (designated as CwIE), a 35‐kDa protein (designated as CwlF) and its activity were also found. In contrast to CwlE production, CwlF production was unaffected by a flaDl ( sinR ) point mutation which represses other vegetative phase autolysins. These newly identified autolysin activities quickly disappeared when cell growth entered stationary phase. The introduction of a sigD ‐null mutation caused the disappearance of Cw1E activity but Cw1F activity was unaffected by the mutation, as judged on zymography. The possible roles of CwlE and CwlF during vegetative growth are discussed.