Open AccessContributions of Xy1R, CcpA and HPr to catabolite repression of the xyl operon in Bacillus subtilis
Author(s) -
Dahl Michael K.,
Hillen Wolfgang
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07814.x
Subject(s) - catabolite repression , ccpa , fed batch culture , bacillus subtilis , operon , psychological repression , repressor , lac operon , biochemistry , biology , chemistry , genetics , transcription factor , gene , bacteria , mutant , gene expression , fermentation
The xyl operon of Bacillus subtilis is regulated at the level of transcription by xylose induction via the Xyl repressor and by catabolite repression. We have investigated the influence of ccpA, ptsH, ptsG and xylR mutations on catabolite repression of xylA expression. The results indicate that full glucose repression of the xyl operon requires CcpA, Hpr and XylR. In contrast, fructose repression depends on CcpA and Hpr, but not on XylR. The ptsH1 mutation relieves catabolite repression only partially, suggesting the possibility that other presently unknown signals are sensed by CcpA.