Open AccessFts insertional mutant of Salmonella typhimurium
Author(s) -
Cerquetti M. Cristina,
Brawer Rolando,
Gerdes Christian A.,
Gherardi M. Magdalena,
Sordelli Daniel O.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07813.x
Subject(s) - salmonella , mutant , microbiology and biotechnology , insertional mutagenesis , biology , genetics , bacteria , gene
A temperature‐sensitive filamentation ( fts ) Salmonella typhimurium mutant was isolated after transposon mutagenesis with mini‐Tn 10d Tc. The mutant was unable to form colonies after 20 h incubation at 37°C on LB agar. Colonies appeared, however, after longer incubation at the restrictive temperature. Filamentation affected only part of the bacterial population. Rapid mapping using Mu d P22 hybrid phages revealed that the mutation, ftsD 220, lies within minutes 68.5 and 73.6 on the genetic map. Further analysis revealed that the ftsD 220 mapped at min 73 and that it is linked to cysG (6%) and to aroB (39%). Complementation tests suggested that the ftsD 220 mutation is not homologous to a Escherichia coli ftsH mutation