Open AccessLipopolysaccharides from six strains of Acetobacter diazotrophicus
Author(s) -
Fontaine Thierry,
Stephan Marilia Penteado,
Debarbieux Laurent,
Previato JoséOsvaldo,
MendonçaPréviato Lucia
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07808.x
Subject(s) - hexose , rhamnose , galactose , chemistry , acetobacter , biochemistry , mannose , arabinose , glucosamine , xylose , chromatography , fermentation , enzyme
Lipopolysaccharides from six nitrogen‐fixing strains of Acetobacter diazotrophicus (PR2, PAL3, PAL5, PR4, PR14, PR20), isolated from sugarcane, were purified by phenol‐water extraction and ultracentrifugation. The relatively large molecular mass observed by SDS‐PAGE indicated that the lipopolysaccharides of each strain possessed an O‐side chain. Analysis of each lipopolysaccharide by colorimetric assays and by gas liquid chromatography/mass spectrometry combination showed that the core and lipid A composition was similar for all strains, containing 3‐deoxy‐d‐manno‐2‐octulosonic acid, glucosamine and fatty acid (16‐0, 3‐OH‐14, 2‐OH‐16:0, 3‐OH‐16:0). The neutral sugar composition showed the predominance of 6‐deoxy‐hexose (rhamnose and fucose) and ribose, in comparison with hexose (glucose, galactose, mannose). The presence of 6‐deoxy‐hexose and ribose containing O‐side chains is discussed as a way of discriminating A. diazotrophicus from other Acetobacter species.