Open AccessA hydroxymethylglutaryl‐CoA reductase inhibitor synthesized by yeasts
Author(s) -
GundeCimerman Nina,
Plemenitaš Ana,
Cimerman Aleksa
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07807.x
Subject(s) - reductase , hydroxymethylglutaryl coa reductase , chinese hamster ovary cell , yeast , biochemistry , hmg coa reductase , chromatography , chemistry , enzyme , microsome , high performance liquid chromatography , pichia , biology , pichia pastoris , recombinant dna , receptor , gene
Screening of different yeast species showed that they are able to synthesize hydroxymethylglutaryl‐CoA (HMGCoA) reductase inhibitors. Crude methanol extracts and the purified inhibitors from Pichia labacensis and Candida cariosilignicola were tested for their biological activity on the solubilized microsomal HMGCoA reductase from Chinese hamster ovary cells. Identification of the inhibitors was studied by thin layer chromatography, high pressure liquid chromatography and mass spectroscopy.