Open AccessProton‐dependent kinetics of citrate uptake in growing cells of Lactococcus lactis subsp. lactis bv. diacetylactis
Author(s) -
Cachon Rémy,
Daniel Sidonie,
Diviès Charles
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07794.x
Subject(s) - lactococcus lactis , kinetics , permease , divalent , biovar , chemistry , biochemistry , ternary complex , substrate (aquarium) , enzyme kinetics , biophysics , biology , bacteria , enzyme , escherichia coli , lactic acid , organic chemistry , active site , gene , genetics , physics , quantum mechanics , ecology
The kinetic analysis of citrate uptake in growing cells of Lactococcus lactis subsp. lactis biovar. diacetylactis identified a proton‐dependent transport and suggested the divalent anionic species as the form of citrate transported across cell membranes. The reaction followed Michaelis‐Menten kinetics for a two‐substrate reaction. The limiting steps were the formation of the ternary complex and the rate of transport. Temperature modified the activity of the permease, increasing the uptake rate.