Open AccessThe use of PCR to isolate a putative ABC transporter from Saccharopolyspora erythraea
Author(s) -
O'Neill Mary P.,
Anne Eady E.,
Radford Alan,
Baumberg Simon,
Cove Jonathan H.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07776.x
Subject(s) - tylosin , oleandomycin , atp binding cassette transporter , biology , spiramycin , gene , microbiology and biotechnology , erythromycin , genetics , homology (biology) , gene cluster , transporter , antibiotics
A gene ( ertX ) encoding a putative ABC transporter was cloned from the erythromycin producer Saccharopolyspora erythraea , using PCR. The primers were based on regions of homology from ABC transporters which confer resistance to macrolide antibiotics. While ertX encodes a protein with a strong degree of similarity to other macrolide ABC transporters from streptomycetes and staphylococci, it did not confer resistance to erythromycin, tylosin, spiramycin, oleandomycin, josamcin, chalcomycin or midecamycin when subcloned into sensitive streptomycete hosts. Southern blot analysis suggested that ertX did not constitute part of the erythromycin gene cluster as identified to date.