Open AccessIsolation of promoters from Brevibacterium flavum strain MJ233C and comparison of their gene expression levels in B. flavum and Escherichia coli
Author(s) -
Zupancic Thomas J.,
Kittle Joseph D.,
Baker Beth D.,
Miller Courtney J.,
Palmer Donna T.,
Asai Yoko,
Inui Masayuki,
Vertès Alain,
Kobayashi Miki,
Kurusu Yasurou,
Yukawa Hideaki
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07765.x
Subject(s) - promoter , biology , transposable element , shuttle vector , escherichia coli , reporter gene , gene , microbiology and biotechnology , genetics , brevibacterium , bacteria , gene expression , vector (molecular biology) , mutant , recombinant dna , microorganism
A promoter probe shuttle vector suitable for the isolation of promoter elements from coryneform bacteria was constructed. This vector carried the neomycin phosphotransferase (NPTII) gene from transposon Tn 5 as a reporter gene, and was capable of replication in both Escherichia coli and Brevibacterium flavum . The vector was used in the construction of a B. flavum library of 899 independently isolated promoter clones. Promoters with a wide range of activities in B. flavum , including some very strong promoter elements, were isolated. Comparative analysis suggests that significant differences between B. flavum and E. coli may exist in the determinants of promoter strength.