Open AccessStrain typing of Lentinula edodes by random amplified polymorphic DNA assay
Author(s) -
Zhang Yinfang,
Molina Francis I.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07747.x
Subject(s) - lentinula , rapd , biology , mushroom , typing , strain (injury) , dna , genetics , microbiology and biotechnology , polymerase chain reaction , genetic marker , gene , food science , genetic diversity , population , demography , anatomy , sociology
Single 10‐base primers were used to generate randomly amplified polymorphic DNA (RAPD) markers in the shiitake mushroom, Lentinula edodes . Seven primers produced polymorphisms in all 15 strains tested, producing 12–19 bands ranging from 0.34 to 2.52 kb. Thirteen of the 15 strains had unique DNA fingerprints, whereas L. edodes ATCC 28759 and ATCC 28760 exhibited identical RAPD profiles for all the primers. Molecular‐genetic markers obtained with the RAPD assay can be used to differentiate strains of L. edodes and have potential applications in mushroom breeding and strain improvement programs.