Open AccessGenomic species typing of acinetobacters by polymerase chain reaction amplification of the recA gene
Author(s) -
Nowak A.,
Kur J.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07739.x
Subject(s) - biology , restriction fragment length polymorphism , genetics , polymerase chain reaction , acinetobacter calcoaceticus , acinetobacter , genomic dna , restriction enzyme , gene , typing , dna , neisseria , terminal restriction fragment length polymorphism , neisseria gonorrhoeae , microbiology and biotechnology , amplified fragment length polymorphism , gene duplication , neisseriaceae , bacteria , genetic diversity , population , demography , sociology
The recA gene has been used as a target in screening for the presence of acinetobacters on the genospecies level and differentiation of relevant acinetobacter species from one another by PCR. Primers deduced from known recA gene sequences of Acinetobacter calcoaceticus and Neisseria gonorrhoeae allowed the amplification of DNAs from all Acinetobacter genospecies. The size of the amplified DNA fragment from all genospecies tested was approximately 435–500 bp relative to DNA size markers. The amplified products were examined further by restriction fragment length polymorphism (RFLP) analysis. Restriction analysis with only two enzymes, Mbo I and Hin fI, enabled us to identify all known genospecies. Since this method uses conserved recA gene sequences for primers, it is expected to be applicable for the identification of most bacterial species.