Open AccessDistribution of individual cytoplasmic pH values in a population of the yeast Saccharomyces cerevisiae
Author(s) -
Cimprich Petr,
Slavík Jan,
Kotyk Arnošt
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07727.x
Subject(s) - saccharomyces cerevisiae , yeast , chemistry , population , distribution (mathematics) , biochemistry , mathematics , sociology , demography , mathematical analysis
Fluorescence ratio imaging microscopy using pH‐sensitive fluorescent dyes makes it possible to evaluate statistical distribution of intracellular pH in a population of the yeast S. cerevisiae examined in a thin layer of suspension in a Petri dish. The distribution appears to fit a Gaussian curve with a half‐width around the 0.4 pH unit. The curve became slightly narrower after resuspension in a strong buffer; the mean values shifted with the pH of the buffer. The shape of the distribution curves of both resting and growing cells in various phases of growth does not change significantly. Likewise, addition of 1% of glucose, 50 μM suloctidil or 100 μM diethylstilbestrol brings about no alteration. The only value which clearly changes is the average cytoplasmic pH.