Open AccessThe role of endonucleases in the expression of ribonuclease II in Escherichia coli
Author(s) -
Zilhão Rita,
Régnier Philippe,
Maria Arraiano Cecília
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07726.x
Subject(s) - rnase p , ribonuclease , rnase mrp , ribonuclease iii , rnase h , escherichia coli , biology , messenger rna , endonuclease , gene expression , microbiology and biotechnology , rna , enzyme , gene , biochemistry , rna interference
Ribonuclease II (RNase II), encoded by the rnb gene, is one of the two major Escherichia coli exonucleases involved in mRNA degradation. Some of the ribonucleases implicated in this process have recently been shown to be inter‐regulated. In this paper we studied the effects of the endonucleases RNase E and RNase III in rnb expression. We have shown that RNase E cleaves the rnb message internally: when this ribonuclease is inactivated rnb mRNA accumulates with a concomitant increase in RNase II activity. RNase III also affects RNase II expression but in an indirect way. We discuss these implications for the regulation of mRNA degradation.