Open AccessDetection by multiplex polymerase chain reaction and typing of Chlamydia trachomatis isolates
Author(s) -
Valassina Marcello,
Cusi Maria Grazia,
Corsaro Daniele,
Buffi Carlo,
Piazzesi Giovanna,
Valensin Pier Egisto
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07721.x
Subject(s) - chlamydia trachomatis , multiplex polymerase chain reaction , polymerase chain reaction , multiplex , biology , typing , serotype , chlamydiaceae , plasmid , chlamydia , population , virology , chlamydiales , microbiology and biotechnology , dna , gene , genetics , medicine , environmental health
The multiplex polymerase chain reaction (PCR) was applied for the detection of the Chlamydia trachomatis chromosome and plasmid. The multiplex PCR demonstrated a sensitivity of 0.8 fg of chlamydial DNA, corresponding to the detection of about 5 copies of the plasmid. Analysis of 195 genital specimens collected randomly from a female population, showed that the multiplex PCR is more sensitive and rapid than culturing for detecting Chlamydia trachomatis . Moreover, sequencing of the II variable domain of the ompl gene, directly from DNA of the clinical specimens, appears to be a simple and rapid method for determining serovar isolates.