Open AccessNAD + ‐glycohydrolase from Streptococcus pyogenes shows cyclic ADP‐ribose forming activity
Author(s) -
Karasawa Tadahiro,
Takasawa Shin,
Yamakawa Kiyotaka,
Yonekura Hideto,
Okamoto Hiroshi,
Nakamura Shinichi
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07720.x
Subject(s) - nad+ kinase , streptococcus pyogenes , cyclic adp ribose , nicotinamide , enzyme , ribose , biochemistry , hydrolysis , cleavage (geology) , chemistry , stereochemistry , sepharose , biology , bacteria , staphylococcus aureus , fracture (geology) , paleontology , cd38 , stem cell , cd34 , genetics
NAD + ‐glycohydrolase from Streptococcus pyogenes was purified by successive chromatography on CM Sepharose CL‐6B, Sephacryl S‐200 HR and hydroxyapatite. The purified enzyme possessed synthesis and hydrolysis activities of cyclic ADP‐ribose (cADPR), a newly found second messenger for Ca 2+ mobilisation, along with cleavage activity of the ribose‐nicotinamide bond in NAD + .