Open AccessEffects of mutations and deletions on expression of the Enterobacter cloacae ompX gene
Author(s) -
Kort Gijs,
Der BentKlootwijk Paola,
Boxtel Renée A.J.,
Klundert Jos A.M.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07699.x
Subject(s) - coding region , signal peptide , biology , enterobacter cloacae , virulence , genetics , cytoplasm , peptide sequence , bacterial outer membrane , gene , complementation , microbiology and biotechnology , enterobacteriaceae , escherichia coli , mutant
The Enterobacter cloacae outer membrane protein OmpX is involved in resistance to β‐lactams, and possesses virulence characteristics. To gain more insight into the genetic elements that are important for OmpX expression, several mutations were introduced at, and immediately upstream of, the N‐terminus of the OmpX coding sequence. These mutations enabled us to delete the 5′ untranslated region and the signal peptide coding sequence. The former led to decreased ompX expression, indicating an unexpected and hitherto unexplained role for this region. Deletion of the signal peptide coding sequence blocked transport across the cytoplasmic membrane, indicating that translocation of OmpX across the cytoplasmic membrane is mediated by the general secretory pathway.