Open AccessDevelopment of two PCR assays for the identification of mycoplasmas causing contagious agalactia
Author(s) -
Dedieu Laurence,
Mady Viviane,
Lefevre PierreCharles
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07587.x
Subject(s) - mycoplasma mycoides , biology , polymerase chain reaction , microbiology and biotechnology , mollicutes , strain (injury) , mycoplasma , gene , genetics , anatomy
A new detection test for the mycoplasmas causing contagious agalactia, Mycoplasma agalactiae , M. capricolum subsp. capricolum and M. mycoides subsp. mycoldes L.C., was developed. It was based on two polymerase chain reaction assays: the Ma‐PCR for the detection of M. agalactiae and the MYC‐PCR for the ‘mycoides cluster’ thus including M. capricolum subsp. capricolum and M. mycoides subsp. mycoides L.C. An M. agalactiae strain was identified by a 933‐bp Ma‐PCR product and no amplification with the MYC‐PCR. In contrast, a 460‐bp MYC‐PCR product and a negative or a 350‐bp Ma‐PCR product characterized a ‘mycoides cluster’ strain. M. capricolum subsp. capricolum and M. mycoides subsp. mycoides L.C. were identified by their species‐specific Asel pattern of the 460‐bp MYC‐PCR product.