Open AccessUnbudded G 2 as well as Gj arrest in the stationary phase of the basidiomycetous yeast Cryptococcus neoformans
Author(s) -
Takeo Kanji,
Tanaka Reiko,
Miyaji Makoto,
Nishimura Kazuko
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07585.x
Subject(s) - cryptococcus neoformans , propidium iodide , dapi , biology , yeast , stationary phase , dna , staining , cryptococcus , microbiology and biotechnology , cell cycle , cell , chemistry , apoptosis , biochemistry , programmed cell death , genetics , chromatography
Stationary‐phase cells of Cryptococcus neoformans displayed two morphological characteristics: virtually all the cells were unbudded even in the early stationary phase and even when grown in rich media, and average cell size increased from that of exponential‐phase cells. DNA contents for small and large stationary‐phase cells were determined by quantitative fluorescence microscopy after DNA staining with propidium iodide or DAPI. Small cells contained G, DNA, whereas large unbudded cells had either a G 2 or G 1 DNA content, indicating that Cr. neoformans can enter into the stationary phase from either the G 1 or G 2 period.