Open AccessPhylogeny of the genus Azospirillum based on 16S rDNA sequence
Author(s) -
Fani Renato,
Bandi Claudio,
Bazzicalupo Marco,
Teresa Ceccherini Maria,
Fancelli Silvia,
Gallori Enzo,
Gerace Letizia,
Grifoni Annamaria,
Miclaus Nerino,
Damiani Giuseppe
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07579.x
Subject(s) - phylogenetic tree , biology , phylogenetics , genus , 16s ribosomal rna , proteobacteria , evolutionary biology , botany , genetics , bacteria , gene
The 16S rDNA of 17 strains of Azospirillum , 14 assigned to one of the known species A. amazonense A. brasilense A. halopraeferens A. irakense and A. lipoferum , and the other three of uncertain taxonomic position, was sequenced after polymerase chain reaction amplification and analysed in order to investigate the phylogenetic relationships at the intra‐generic and super‐generic level. The phylogenetic analysis confirms that the genus Azospirillum constitutes a phylogenetically separate entity within the a subclass of Proteobacteria and that the five species are well defined. A. brasilense and A. lipoferum are closely related species and form one cluster together with A. halopraeferens ; the pair of species A. amazonense and A. irakense forms a second cluster in which Rhodospirillum centenum is also placed.