Open AccessNon‐reciprocal regulation of Rhodobacter capsulatus and Rhodobacter sphaeroides recA genes expression
Author(s) -
Henestrosa Antonio R.Fernandez,
Rivera Eusebi,
Barbé Jordi
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07576.x
Subject(s) - rhodobacter , rhodobacter sphaeroides , repressor lexa , biology , gene , escherichia coli , genetics , microbiology and biotechnology , gene expression , bacteria , mutant , repressor
The Rhodobacter capsulatus recA gene has been isolated and sequenced. Its deduced amino acid sequence showed the closest identity with the Rhodobacter sphaeroides RecA protein (91% identity). However, the promoter regions of both R. capsulatus and R. sphaeroides recA genes are only 64% similar. An Escherichia coli ‐like LexA binding site was not present in the upstream region of the R. capsulatus recA gene. Nevertheless, the R. capsulatus recA gene is inducible by DNA damage in both hetero‐ and phototrophically growing conditions. The R. capsulatus recA gene is poorly induced when inserted into the chromosome of R. sphaeroides , indicating that the recA gene of both bacteria possess different control sequences despite their phylogenetically close relationship.