Open AccessConstruction and preliminary characterisation of mini‐derivatives of a large (107‐kb) cryptic plasmid of the sulfur bacterium Thiobacillus versutus
Author(s) -
Bartosik Dariusz,
Baj Jadwiga,
Wlodarczyk Miroslawa
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07575.x
Subject(s) - replicon , plasmid , biology , escherichia coli , genome , microbiology and biotechnology , genetics , dna , gene
Several mini‐replicons, derivatives of a large (107‐kb) cryptic Thiobacillus versutus pTAV1 plasmid, were obtained. The pTAV1 derivatives confer all functions sufficient for autonomous replication in T. versutus but they cannot be maintained in Escherichia coli . The fragment of pTAV1 (4‐kb) included in the smallest mini‐replicon, pTAV202, encodes for two proteins of approximately 26 and 45 kDa. The region responsible for stable maintenance of pTAV1 derivatives (and presumably entire pTAV1) was located in defined 14‐kb fragment of pTAV1 genome. Hybrid plasmids composed of E. coli vectors (pBGS18 or pWSK29) and pTAV202 replicon were constructed and their activity in both hosts tested.