Open AccessLack of lactate‐proton symport activity in pck1 mutants of Saccharomyces cerevisiae
Author(s) -
Casai Margarida,
Blázquez Miguel A.,
Gamo Franciscojavier,
Gancedo Carlos,
Leão Cecília
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07536.x
Subject(s) - symporter , derepression , biochemistry , mutant , saccharomyces cerevisiae , chemistry , phosphoenolpyruvate carboxykinase , allosteric regulation , lactic acid , yeast , biology , enzyme , bacteria , transporter , gene , psychological repression , gene expression , genetics
Mutants of Saccharomyces cerevisiae without phosphoenolpyruvate carboxykinase activity showed no measurable lactate proton symport, while mutants without fructose‐1,6‐bisphosphatase had normal transport activity. Incubation of a pck1 mutant, under derepression conditions in the presence of glycerol, restored the activity of the lactate‐proton symport, with identical kinetic characteristics to that in the wild‐type. For efficient lactate‐proton symport activity, not only is an external inducer such as lactic acid needed, but also a molecule derived from the acid metabolism may be necessary.