Open AccessCloning and sequencing of a novel hemolysis gene of Vibrio cholerae
Author(s) -
Nagamune Kisaburo,
Yamamoto Koichiro,
Honda Takeshi
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07534.x
Subject(s) - vibrio cholerae , hemolysis , biology , molecular cloning , gene , microbiology and biotechnology , cloning (programming) , escherichia coli , lysis , vibrionaceae , el tor , bacteria , peptide sequence , genetics , computer science , immunology , programming language
A hemolysis gene ( hlx ) which lyses sheep erythrocytes on blood agar plates when expressed in Escherichia coli was cloned from Vibrio cholerae . The cloned gene is predicted to encode a polypeptide of 92 amino acid residues with a deduced molecular mass of 10451. E. coli transformed with this gene lysed sheep, goose, horse and chicken erythrocytes but not those of guinea pig and human. The hlx gene was observed in classical‐ and El Tor‐biotype V. cholerae O1, V. cholerae non‐O1, and V. mimicus , but not in V. parahaemolyticus .