Open AccessA rapid permeabilization procedure for accurate quantitative determination of β‐galactosidase activity in yeast cells
Author(s) -
Kippert Fred
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07523.x
Subject(s) - yeast , schizosaccharomyces pombe , saccharomyces cerevisiae , incubation , biochemistry , chromatography , biology , chemistry
A procedure is described which allows the rapid permeabilization of yeast cells, Schizosaccharomyces pombe and Saccharomyces cerevisiae , for quantitative in situ assays of β‐galactosidase activity. Yeast cells are permeabilized by incubation in buffer containing 0.2% of the detergent sodium lauroyl sarcosinate without any need for washing or vortexing. This procedure is equally applicable to fresh and frozen samples. It is compared to earlier reported methods and found to be superior by being more accurate and less time‐consuming.