Open AccessApplication of random amplified polymorphic DNA (RAPD) assays in identifying conserved regions of actinomycete genomes
Author(s) -
Mehling Annette,
Wehmeier Udo F.,
Piepersberg Wolfgang
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07510.x
Subject(s) - biology , rapd , primer (cosmetics) , genetics , genomic dna , intergenic region , genome , 23s ribosomal rna , dna , dna sequencing , gene , microbiology and biotechnology , ribosomal rna , polymerase chain reaction , multiple displacement amplification , rna , dna extraction , chemistry , ribosome , population , demography , organic chemistry , sociology , genetic diversity
Various arbitrary primers as well as pUC18/19 ‘reverse’ sequencing primers were used for random amplified polymorphic DNA assays. Use of a modified reverse primer led to amplification of one major approx. 1100‐bp band from the chromosomal DNA of all actinomycetes tested; however, the band was not found when DNAs from other bacteria were used in comparable experiments. Hybridization experiments showed that these bands all contained similar genomic regions. Subsequent sequencing of four of these fragments showed they each contained the sequence of the 3′ end of the 23S rRNA gene, the intergenic region and the start of the 5S rRNA gene.