Open AccessEffect of nutrient nitrogen and manganese on manganese peroxidase and laccase production by Lentinula (Lentinus) edodes
Author(s) -
Buswell John A.,
Cai Yijin,
Chang Shuting
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07504.x
Subject(s) - lentinula , lentinus , laccase , manganese peroxidase , peroxidase , chemistry , food science , manganese , substrate (aquarium) , lignin peroxidase , lignin , enzyme , biochemistry , nuclear chemistry , mushroom , organic chemistry , biology , ecology
Lentinula (Lentinus) edodes , strain LS4, produces manganese‐dependent peroxidase (MnP) and laccase, but not lignin peroxidase, when grown on a defined medium with glucose as sole carbon source. MnP production is suppressed by nitrogen whereas highest levels of laccase were observed when the fungus was grown under high nitrogen (26 mM) conditions. Both the titre and time of appearance of MnP were affected by the concentration of Mn in the culture medium with highest enzyme levels recorded in cultures supplemented with 1.1 ppm Mn. Purified MnP from L. edodes LS4 has an apparent M r of 59000 and a p I of 5.6, and differs in several respects from a MnP isolated from L. edodes grown on a commercial wood substrate.