Open AccessMycoplasmas regulate HIV‐LTR‐dependent gene expression
Author(s) -
Nirpaz Ran,
Israel Shoshana,
Honigman Alik,
Kahane Itzhak
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07501.x
Subject(s) - jurkat cells , biology , transcription (linguistics) , transfection , gene , long terminal repeat , reporter gene , plasmid , ureaplasma urealyticum , gene expression , hiv long terminal repeat , virology , mycoplasma , microbiology and biotechnology , t cell , genetics , linguistics , philosophy , immune system
Mycoplasmas have been incriminated in setting the stage for HIV infection and full‐blown AIDS. We tested the possible involvement of mycoplasmas in activation of HIV. Two cell lines, 293 fibroblasts and Jurkat CD4+ T‐cells, transfected with plasmids harboring a transcription fusion construct between HIV‐long terminal repeat (HIV‐LTR) and either luc or cat genes, were infected with several mycoplasmas ( M. fermentons; M. penetrons M. pirum and Ureaplasma urealyticum ) and the reporter gene expression was monitored. The data presented here suggest that mycoplasmas, and specifically their membranes, play a role in the activation of HIV‐LTR mediated transcription.