Open AccessConstruction of a food‐grade host/vector system for Lactococcus lactis based on the lactose operon
Author(s) -
Maccormick Caroline A.,
Griffin Hugh G.,
Gasson Michael J.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07457.x
Subject(s) - lactococcus lactis , plasmid , operon , biology , lactose , gene , strain (injury) , vector (molecular biology) , genetics , microbiology and biotechnology , bacteria , recombinant dna , biochemistry , escherichia coli , lactic acid , anatomy
A plasmid‐based food‐grade vector system was developed for Lactococcus lactis by exploiting the genes for lactose metabolism. L. lactis MGS267 is a plasmid‐free strain containing the entire lactose operon as a chromosomal insertion. The lacF gene was deleted from this strain by a double cross‐over homologous recombination event. The lacF ‐deficient strain produced a Lac − phenotype on indicator agar. A cloned copy of the lacF gene expressed on a plasmid was capable of complementing the lacF ‐deficient strain resulting in a Lac + phenotype. This stably maintained system fits the requirements of a self‐selecting vector system and has the potential to be exploited in the food industry.