Open AccessA serotype‐specific epitope of dengue virus 1 identified by phage displayed random peptide library
Author(s) -
Yao ZhiJian,
Kao Mandy C.C.,
Loh KeanChong,
Chung Maxey C.M.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07455.x
Subject(s) - epitope , antigenicity , dengue virus , virology , dengue fever , monoclonal antibody , serotype , linear epitope , epitope mapping , mimotope , biology , peptide sequence , peptide library , peptide , phage display , antibody , microbiology and biotechnology , biochemistry , genetics , gene
From a panel of monoclonal antibodies of dengue viruses, a serotype‐specific epitope of dengue virus 1 was screened from a random peptide library displayed on phage. The epitope was the determinant reactive with monoclonal antibody 15F3‐1 that was specific to dengue 1. The screening was monitored by a dot blotting procedure, and after three rounds of screening a consensus motif, HRYSWK, was found. This sequence matches the sequence HKYSWK, corresponding to the amino acid residues 885–890 of polyprotein or residues 111–116 of the non‐structural protein 1 of dengue virus serotype 1. The linear epitope was confirmed by testing the antigenicity of chemically synthesized 8‐branched peptide.