Open AccessDefective sterol Δ 5(6) desaturase as a cause of azole resistance in Ustilago maydis
Author(s) -
Josephhome Timothy,
Manning Nigel J.,
Hollomon Derek,
Kelly Steven L.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07445.x
Subject(s) - ustilago , sterol , azole , biology , microbiology and biotechnology , strain (injury) , alpha (finance) , ergosterol , biochemistry , antifungal , cholesterol , gene , anatomy , medicine , construct validity , nursing , patient satisfaction
Resistance to azole antifungals in Ustilago maydis was associated with a leaky defect in sterol Δ 5(6) desaturase. This defect resulted in reduced accumulation of 14α‐methylergosta‐24(28)‐diene‐3β, 6 α‐diol and an increase in the proportion of 14α‐methylfecosterol in treated cells when compared to the parent strain. The results demonstrate the importance of this mechanism in pathogenic fungi.