Open AccessProduction of Escherichia coli LamB protein in Yersinia enterocolitica
Author(s) -
Brzostek Katarzyna,
Heleszko Halina,
Hrebenda Jerzy
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07443.x
Subject(s) - yersinia enterocolitica , microbiology and biotechnology , biology , chemistry , bacteria , genetics
Plasmid pBCP 68 carrying the lamB gene of Escherichia coli was introduced and expressed in Yersinia enterocolitica cells. The presence of LamB protein in the outer membrane of the wild‐type strain of Y. enterocolitica coincided with the loss of the OmpC and OmpF porins. Western blot analysis showed that LamB in Y. enterocolitica cells co‐migrated with authentic monomeric LamB, indicating that its signal peptide was recognized and cleaved by Y. enterocolitica and properly integrated into the outer membrane. The expression of LamB made Y. enterocolitica sensitive to phage λ.