Open AccessStudies on lipopeptide biosynthesis by Bacillus subtilis : Isolation and characterization of iturin − , surfactin + mutants
Author(s) -
Feignier Christine,
Besson Françoise,
Michel Georges
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07442.x
Subject(s) - surfactin , bacillus subtilis , lipopeptide , mutant , biosynthesis , strain (injury) , chemistry , bacillaceae , bacillales , biochemistry , wild type , moiety , microbiology and biotechnology , biology , bacteria , stereochemistry , enzyme , gene , genetics , anatomy
Two mutant strains, M35 and M89, were obtained by UV irradiation from a wild‐type Bacillus subtilis producing iturin and surfactin. Sporulation and surfactin production were similar in both mutants and in the parent strain, while the iturin production of M35 was 300‐fold less than that of the wild‐type strain; M89 did not produce any iturin. The analysis of the incorporation of sodium [1‐ 14 C]acetate into cellular lipids and lipopeptides showed that M89 still synthesized β‐amino fatty acids, the lipid moiety of iturin.