Open AccessEvidence for the existence of isocitrate lyase activity in methylotrophic Hyphomicrobium strains
Author(s) -
Yoshida Toyokazu,
Tanaka Yasuhiro,
Hagishita Tairo,
Mitsunaga Toshio,
Izumi Yoshikazu
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07422.x
Subject(s) - isocitrate lyase , dithiothreitol , biochemistry , enzyme , serine , lyase , chemistry , methylotroph , biology , glyoxylate cycle
Isocitrate lyase activities were detected in a range of 0.096–0.212 units mg −1 in cell‐free extracts of all tested Hyphomicrobium strains grown on methanol as a sole carbon source, although the activities were rapidly lost during storage at 4 °C. When cell‐free extracts were incubated with dithiothreitol, after storage the recovery of activity was observed, indicating the involvement of a labile sulfhydryl group in the enzyme. This confirmed the distribution of unstable isocitrate lyase in the genus Hyphomicrobium , and, contrary to previous observations, the operation of the ic + ‐serine pathway was suggested for the assimilation of one‐carbon compounds.